Spectramax m series multimode microplate readers biothing. The turbidity test uses a spectramax plus 384 microplate reader to measure the absorbance of uv light wavelengths 320 and 600 nm to ensure turbidity changes being recorded by either. Engineering platforms for directed evolution of laccase from. The washer is engineered to be simple to maintain and use, including features that reduce clogging and noise. Galcer is a synthetic nkt cellspecific ligand presented exclusively to invariant nkt cells and is known to improve immunity against tumors and infection by promoting ifn. Softmax pro software is designed to provide the simplicity, flexibility and power required for advanced data analysis. Chemical shifts of proton 1 h nmr and carbon c nmr were reported in ppm relative to the residual solvent peaks except that methanol was employed as the external. Nov 25, 2014 oxidative stressinduced damage to retinal pigmented epithelial rpe cells is implicated in the progression of agerelated macular degeneration amd, which is one of the primary causes of vision loss in the elderly. Fluorescence plate reader molecular device, spectramax geminixs 1 turn on the instrument spectramax geminixs and allow the instrument for selfcalibration and initialization. The absorbance at the wavelength of 486 nm was recorded by using a spectramax plus 384 microplate reader molecular devices and the initial rate of nitrocefin hydrolysis reaction was determined by softmax pro 5. The wells were inoculated from overnight cultures of bacteria started from glycerol stocks to produce an initial od 600 nm of 0. Spectramax plus 384 absorbance plate reader, molecular. Details about molecular devices spectramax i3 used. Static microplate fermentation and automated growth analysis.
Ref spectramax plus 384 only a reading of buffer, water, or air taken in the cuvette that. Molecular devices custom module editor software package. Molecular devices uv lamp vmax, emax,thermomax,spectramax. Molecular devices uv lamp vmax, emax,thermomax,spectramax 340, spectramax plus. Spectramax plus 384 microplate reader manual conquer. Interaction of dietary resveratrol with animalassociated. Molecular devices spectramax plus the lab world group.
Molecular devices spectramax 340pc microplate reader. Using this software, the stakmax microplate handling stacker can also be flawlessly integrated with the spectramax plus 384 system. Feb 28, 20 molecular devices, market leader in microplate reader technology for over 25 years, has announced the launch of its spectramax i3 multimode detection platform the system is available as a standalone reader with the option for users to upgrade to additional applications and detection modes such as cellular imaging with the spectramax minimax imaging cytometer, and cartridges for time. Manufacturer specifications spectramax 340 pc, molecular. Skeletal muscle tissue engineering te aims to efficiently repair large congenital and acquired defects. Molecular devices spectramax plus 384 microplate reader is a microplate reader with builtin cuvette port and microplate drawer can run both standard spectrophotometer and microplate.
The introduction of the molecular devices spectramax plus microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm pathlength cuvette. Kpro front plates optical core were functionalized with pmaa and l. Jun 29, 2019 rhodosporidium toruloides is a promising host for the production of bioproducts from lignocellulosic biomass. Oct 14, 2014 triple negative breast cancers tnbcs have a poor prognosis 5y survival of 74. A role for cd1drestricted nkt cells in injuryassociated t cell suppression douglas e. Biological acellular scaffolds are considered a good tool for te, as decellularization allows structural preservation of tissue extracellular matrix ecm and conservation of its unique cytokine reservoir and the ability to support angiogenesis, cell viability, and proliferation. Please read this license agreement carefully before opening the software package. Mutagenic organized recombination process by homologous in. Od 600 was monitored using a spectramax plus 384 spectrophotometer molecular devices, san jose, ca, usa. Use of selfassembling peptides to enhance stem cell function. High performance liquid chromatography hplc analysis. After 3 h of incubation, the solution was placed in 96well plates and observed under enzymelinked immunosorbent assay elisa micro plate reader spectramax plus 384, molecular devices, usa at 450 nm.
Splenocytes were collected and cultured overnight ova 200. Directed in vitro evolution of precambrian and extant rubiscos. Spectramax plus 384 microplate reader molecular devices. The uv absorption spectra of pegaunps were obtained using a spectramax plus 384 absorbance plate reader molecular devices, sunnyvale, ca. Jun 29, 2019 for growth rate experiments, strains were grown in the same conditions as the fluorescence measurement experiments. The spectramax plus 384 by molecular devices is a multifunctional microplate reader. Manager, nanowire assembly nanosys, inc 2002 2009 7 years. The molecular devices spectramax plus is a standard for uvvisible microplate reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96well microplates. Designed for users needing a high quality spectrum analyzer with a minimal learning curve. However, if anything breaks due to a manufacturing defect, we will fix or replace it. The introduction of the molecular devices spectramax plus microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be. Lisa sheth associate research scientist fresenius kabi. Here, we demonstrate the delivery and immunogenicity. The plates were incubated at room temperature in darkness until the color.
If no reference reading is taken, the instrument uses the i 0. Molecular devices imagexpress micro xls system with warranty. Pdf mutagenic organized recombination process by homologous. A key prerequisite for efficient pathway engineering is the availability of robust genetic tools and resources. Use any standard cuvette, 12 x 75 mm test tube, 96 or 384well microplate. Spectramax plus 384 microplate spectrophotometer operators manual iii this warranty only covers defects arising under normal usage and does not cover malfunctions of failures. Molecular devices spectramax plus 384 microplate reader. Spectramax m series multimode microplate readers multimode olvasok with six different models to choose from, its easy to find the one you want for your lab every spectramax m series multimode microplate reader does uv and visible absorbance, and fluorescence intensity measurements on microplates and cuvettes, standard. The molecular devices spectramax 340pc microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Molecular devices spectramax 384 plus microplate reader. See the complete profile on linkedin and discover james connections and jobs at similar companies.
Production of ent kaurene from lignocellulosic hydrolysate. Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the spectramax m2 and spectramax m2e. We show that intercellular adhesion molecule1 icam1 is differentially expressed in human tnbc tumor tissues by immunohistochemistry and in human tnbc cell. Samples were taken at 0, 8, 24, 48, 96 and 168 h after inoculation. Please contact us using the methods below or find your local distributor in the search box. Dramatic differences in organophosphorus hydrolase activity. Molecular devices spectramax plus384 microplate reader manual attachments molecular devices spectramax plus384 microplate reader manual 624 kb.
Molecular devices imagexpress micro xls system with. Plates were stirred briefly and the initial absorptions at 418 nm. Molecular device spectramax 340 pc 384well microplate reader. I platebased assay for studying how fungal volatile. Molecular device spectramax plus 384 microplate reader, refurbishedmolecular device spectramax plus 384 uvvisible, absorbancemicroplate reader for 96 and 384 well. The instrument reads any standard cuvette, microcuvette, test tube or 96 and 384 well standard or uv transparent microplate over a full 190 to. The present studies show that sulindac, a known nonsteroidal antiinflammatory drug, can protect an established rpe cell line, lowpassage human fetal rpe, and polarized primary. A toolset of constitutive promoters for metabolic engineering. For the ldh assay, the cells were evaluated at 24 and 48 hours after treatment of 0. After lyophilization, odns were resuspended and the optical density for each was measured at 260 nm using a spectramax 384 plus 96well plate reader. Systems range from simple singlemode readers such as the emax plus absorbance reader, to the spectramax i3x multimode microplate reader, the firms flagship product. Optical density od was determined by measuring absorbance at 600 nm od 600 with a spectramax plus 384 microplate reader molecular devices, plus384, san jose, ca. Spectramax plus 384 spectrophotometer from molecular. Mutagenic organized recombination process by homologous in vivo grouping morphing is a onepot random mutagenic method for short protein regions that harnesses the in vivo recombination.
Signal generation and recordingplayback are also available. Future ready the spectramax i3 x multimode microplate reader measures spectralbased absorbance, fluorescence, and luminescence with the added functionality of modular. Softmax pro can collect data from one or more microplates or cuvettes and. Users will find prewritten max protocols that enable measurement of low volume applications. Measurements of enzyme activity wildtype and hybrid activities were assayed on a spectramax plus 384 spectrophotometer by monitoring the release of pna at 405 nm following enzymatic hydrolysis of the synthetic tetrapeptides 05. Softmax pro users manual iii softmax pro license agreement and warranty important. This premium allinone data software is capable of performing a wide range of readytoread protocols. Nov 03, 2009 the formation of pnitrophenol was followed at a 412. Multiwash microplate washer, 96 and 384well automated.
Absorbance readings were obtained at 490 nm using a spectramax plus 384 microplate reader molecular devices at 1, 2, and 3 h after incubation. Used molecular devices spectramax 340pc microplate. Spectramax plate reader eastern virginia medical school. Knowledge in operating the sysmex, nova phox, spectramax plus 384, facscanto ii, and olympus au400e knowledge in platelet storage, sterility testing, blood filtrations, lymphocyte apoptosis. Every step is optimized for data acquired from a molecular devices microplate reader or data imported from another source to. Ref spectramax plus 384 only a reading of buffer, water, or air taken in the cuvette that is used as i 0 to calculate absorbance or % transmittance. Manufacturer specifications spectramax 340 pc, molecular devices please note. It provides readytorun protocols, analysis algorithms, and 21 different curve fit options. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96 or 384 well microplate.
Growth was quantified with absorbance reading at 600 nm using a microplate reader spectramax plus 384. Phenyl acetate and paraoxon were prepared as high concentration stocks in meoh and diluted into reaction buffer on day of assay. Jan 02, 2015 absorbance was recorded on an agilent cary 100 uvvis spectrophotometer or molecular devices spectramax plus 384. Mgc8 cells were used to determine the specificity of the assay. The company offers microplate readers and washers with a variety of detection modes and fieldupgradability. Molecular devices introduces spectramax i3 multimode. The equipment has a wavelength range of 100 nm with 1. Spectramax plus 384 microplate spectrophotometer operators manual introduction general overview the spectramax plus 384 microplate spectrophotometer provides rapid and sensitive uvvis measurements of a variety of analytes including speci. Icam1 as a molecular target for triple negative breast cancer. Molecular devices spectramax plus 384 mikrotiterplatten. Rational design of a fusion protein to exhibit disulfide. However, there is a lack of characterized promoters to drive expression of heterologous genes for strain engineering in r. Potent induction of b and tcell immunity against human. Elisa plates were read using a spectramax plus 384 plate reader molecular dynamics, sunnyvale, ca, and analyses of elisa data were done with softmax pro.
Approximately 100 ml of the solution was transferred to a 96well plate and measured with a microplate reader spectramax plus 384 reader, molecular devices, usa at 570 nm. The molecular devices spectramax 384 plus is a uvvis spectrophotometer and microplate reader combination for more reliable and fast data reporting with builtin cuvette port and. Jim hamilton manager, mechanical engineering, digital. Spectramax microplate spectrophotometer operators manual.
Dmso and betaine greatly improve amplification of gcrich. Nitrocefin was added to the final concentration of 50. Postsynthesis steps included odn cleavage from the support followed by basedeprotection overnight at 55c with ammonium hydroxide 2830% j. Read one sample or up to 384 at a time its your choice.
The concentration of pegaunps in solution expressed in molar concentration of gold was determined using the optima 7300 inductively coupled plasmaatomic emission spectroscopy icpaes instrument. Molecular devices the spectramax m2 is a dualmonochromator, multidetection microplate reader with a dualmode. None of the equipment posted for sale on is owned by medwow, should you have any. The molecular devices spectramax 384 plus is a uvvis spectrophotometer and microplate reader combination for more reliable and fast data reporting with builtin cuvette port and microplate drawer. Desktop pc with the above software installed and one 27 monitor. Molecular devices introduces microplate reader and washer.
Spectramax plus 384 absorbance plate reader, molecular devices microplate readers this uvvisible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96well, and 384 well microplates, with the only temperatureindependent method for pathlength correction. The spectramax plus 384 microplate reader gives you walkup convenience for both standard spectrophotometer and microplate reader applications on the. The molecular devices spectramax 384 plus uvvis microplate reader is a versatile device which can be used for both standard spectrophotometer and microplate reader applications. Top detection is available for fluorescence detection on the spectramax m2. Molecular device spectramax plus 384 microplate reader. The optical transmission of the pmma disks functionalized and nonfunctionalized was examined by a uv. Managed group of scientists and engineers developing nanowire assembly process for lowcost, highperformance activematrix displays. Pharmacological protection of retinal pigmented epithelial. Fluorescence plate reader molecular device, spectramax. View james omearas profile on linkedin, the worlds largest professional community. Dna vaccines delivered using electroporation ep have had clinical success, but these ep methods generally utilize invasive needle electrodes.
Decellularized diaphragmatic muscle drives a constructive. This spectrophotometer offers optimal flexibility by accommodating a range. It allows you to create your own image analyses modules and algorithms. The plates were then incubated at room temperature until a green abts or yellow nbd color developed. The spectramax plus 384 microplate reader from molecular devices can run both standard spectrophotometer and microplate reader applications on the same instrument.
Spectramax m2m2e user guide university of michigan. The spectramax 190 system the onboard microprocessor calculates and reports the absorbance or % transmittance for each well of a microplate. Molecular devices spectramax384plus microplate reader. Pdf using biomimetic polymers in place of noncollagenous. Spectramax m2 is a dualmonochromator, multidetection microplate reader with a dualmode cuvette port and 6384 microplate reading capability. Dec 30, 2011 the plates were briefly stirred, and the absorption at 418 nm. The depletion assay was followed in kinetic mode 340 nm using a plate reader spectramax plus 384, molecular devices, sunnyvale, ca supplementary figs 1 and 2. Spectramax plus 384 microplate reader from molecular. Nmr spectra were recorded on a varian 400 mhz spectrometer. L of cck8 solution was added into each well of the cell culture plate. Computer aided designing facility supported by proengineering software.
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